Training in IVF and various other Assisted Reproductive Techniques for Gynecologists
Advanced Infertility Training Course of 14 days
1st Week – Complete Andrology know how
2nd Week – ART, Endoscopy, USG
Training program includes:
- IUI Stimulation Protocols
- Fertility enhancing Endoscopic Surgery
- Sonography related to Infertility
- IVF-ICSI Infertility Lab work
- Cryopreservation
- Setting up of ART units
- National Guidelines by ICMR
4 Weeks Fellowship Program of “Advanced Infertility & ART”
Training program includes:
1. Andrology: Here you will have hands on experience of..
- Sperm counting
- Semen processing for IUI/IVF/ICSI/retrieved sperm
- Hands on IUI
- Cryopreservation of sperm & embryo & eggs
- Thawing – sperm banking
2. Embryology-IVF
- You will trained for selecting ideal protocol
- Hands on experience with ovum pick up
- Trained to identify OCC
- Experience to handle unfertile embryos
- How to do ET, embryo loading experience, freezing, thawing & vitrification process
3. USG-Infertility related USG
Ovulation, COH monitoring, Color Doppler, Pulse Doppler studying, early pregnancy, Targeted USG, Color Doppler in Obs. Gyn will be trained with hands on experience
4. OPD
How to take history, which investigations why, how to convince patient for IVF/ICSI, cost effective IVF will be trained on patients and with patients, patient counseling-in failed case
5. Cryofreezing of embryos, thawing, PESA, TESE procedure will be demonstrated.
SIX Months Certification Course in Reproductive Medicine
Syllabus for Certificate Course in Reproductive Medicine
Expectations at the end of the course, the trainee should be able to:-
FEMALE INFERTILITY:
- Take an appropriate history and examine the woman.
- Evaluate, describe, diagnosis and plan therapy for: ovulatory disorders: including use of basal body temperature, plasma progesterone and endometrial biopsy; diagnosis of causes of anovulation: syndromes of inappropriate prolactin secretion, CNS hypothalamic- pituitary syndromes and other causes; selection of ovulation induction utilizing antiestrogens, gonadotrophins, dopamine agonists, GNRH, GNRH analogues and other agents; tubal disorders: including correct use of and interpretation of studies of tubal function (e.g., ultrasound, hysterosalpingography and laparoscopy); indications for tubal reparative procedures including micro-surgery/ or laparoscopic surgery, versus assisted conception; endometriosis and other peritoneal disorders: including diagnosis and staging of endometriosis and other peritoneal causes of infertility; knowledge of the medical management of endometriosis; cervical factors: including tests for sperm/cervical mucus interaction and possible therapy; artificial insemination including the indications and contra-indications; selection of donors and sperm banking; ovum donation: indications, recruitment, counseling and methods for preparation of donors and recipients; adoption: including the indications for adoption; knowledge of appropriate counselling methods; familiarity with various local agencies and legal implications dealing with adoption. Surrogacy: indications, knowledge of appropriate counseling methods.
MALE INFERTILITY:
- The trainee should be able to take an appropriate history and examine the man, including detailed genital examination and arrange /perform appropriate investigations and treatment.
- The trainee should understand and be able to discuss:- the formation and content as well as examination of the seminal fluid; the cycle of spermatogenesis, including endocrinological control mechanisms, its abnormalities and the effects of drugs; the physiology and Pathophysiology of sexual function; causes of azoospermia and aspermia; the biosynthesis of estrogens, androgens and progestogens by the human testis and the biological action of testosterone in man; investigation, diagnosis and therapy of infection of the male reproductive system; cryobiology of semen, counseling of donors and recipients of DI, sperm banking; in vitro and laboratory tests of sperm function e.g., mucus penetration, biochemistry etc.; the value and limitations of testicular biopsy and endocrine assessment such as plasma FSH; the physiology of endocrine and gametogenic function of the testes and accessory glands.
- Indications and methods of assisted fertilization, including intracytoplasmic sperm injection ;Methods of surgical sperm retrieval.
Psychosexual Aspects of Reproductive Medicine Objectives:
The trainee should understand and be able to discuss:-
- The psychodynamics of growth and development, puberty and the establishment of the gender role.
- The psychological changes associated with treatment of infertility.
In-Vitro Fertilisation (IVF) and other Assisted Reproduction Techniques Objectives:
The trainee should be competent for independent clinical practice in:
- Conditions for which IVF and related techniques of assisted reproduction are appropriate.
- Determination of the menstrual cycle to plan synchronization.
- Follicular stimulation and monitoring by ultrasound, steroid and peptide assays.
- The timing of oocyte aspiration, laparoscopic, and ultrasound based procedures.
- In-vitro gamete transport, maturation and fertilization.
- Surgical and non-surgical methods of sperm retrieval and their use in assisted fertilization.
- Timing and methods of embryo transfer.
- Monitoring of implantation.
- Assessment of genetic abnormalities and their potential treatment.
- Relevant aspects of cryobiology.
- Psychological assessment and management of gamete donors and recipients.
Ethical and Legal Aspects of ART
The trainee should be able to discuss the ethical and legal aspects of the clinical practice of their Subspecialty and should have particular knowledge of the relevant areas listed below:-
- Legislation, particularly recent, relevant to their subspecialty practice.
- Ethics of health care provision and resource allocation.
- Medical confidentiality.
- Consent.
1- week introductory Embryology training for beginners in IVF- ICSI and Andrology
- How to set up Andrology Laboratory and what are the equipment required?
- Handling, cleaning, maintenance and calibration of different equipment-Laminar Flow workstation, Microscopes, Centrifuges, etc.
- Comprehensive semen analysis-Macroscopic and Microscopic-density, motility, viability, round cell differentiation etc.
- Sperm morphology assessment according to WHO and Strict (Kruger) criteria.
- Sperm survival test.
- Semen cryopreservation-both neat and processed sample.
- Semen preparation for IUI-Classical method, Standard method and Density gradient method.
2 weeks basic Embryology training for beginners in IVF- ICSI and Andrology
Andrology Laboratory Techniques
- How to set up Andrology Laboratory and what are the equipment required?
- Handling, cleaning, maintenance and calibration of different equipment-Laminar Flow workstation, Microscopes, Centrifuges, etc.
- Comprehensive semen analysis-Macroscopic and Microscopic-density, motility, viability, round cell differentiation etc.
- Sperm morphology assessment according to WHO and Strict (Kruger) criteria.
- Sperm survival test.
- Semen cryopreservation-both neat and processed sample.
- Semen preparation for IUI-Classical method, Standard method and Density gradient method.
Embryology Laboratory Techniques
- How to set up Embryology laboratory-Lab space and layout, equipment, design flooring, HEPA filter etc.
- Monitoring, calibration and maintenance of equipment-measuring and adjustment of carbon dioxide (CO2) & temperature of incubators and cleaning of incubators.
- Embryology lab maintenance-daily, weekly and monthly.
- List and selection of disposables and culture media.
- Preparation of Embryology Lab for oocyte retrieval the day before-checklist.
- Preparation of lab on the day of oocyte retrieval.
- Identification of granulose cells, cumulus and corona cells and oocytes.
- Separation of oocytes and further culture.
- Preparation of semen for insemination of oocytes-processing of ejaculated semen.
- Method of oocyte insemination-short co-incubation, etc.
- Dissection of oocytes and fertilization check.
- Criteria for fertilization check
Day – 1 Fertilization check, classification of zygotes and further culture.
Day 2 – Assessment of embryo cleavage and identification of good embryos
Day 3 – Assessment of cleavage and selection of best quality embryos for transfer
Day 5/6- Blastocyst culture -technique
Embryo Transfer
- Preparation for embryo transfer.
- Selection of embryos for transfer- day 2, day3 or day5.
- Technique of embryo loading into the catheter (mock transfer).
4 weeks comprehensive Embryology training for beginners in IVF- ICSI and Andrology
Andrology Laboratory Techniques
- How to set up Andrology Laboratory and what are the equipment required?
- Handling, cleaning, maintenance and calibration of different equipment-Laminar Flow workstation, Microscopes, Centrifuges, etc.
- Comprehensive semen analysis-Macroscopic and Microscopic-density, motility, viability, round cell differentiation etc.
- Sperm morphology assessment according to WHO and Strict (Kruger) criteria.
- Sperm survival test.
- Semen cryopreservation-both neat and processed sample.
- Semen preparation for IUI-Classical method, Standard method and Density gradient method.
Embryology Laboratory Techniques
- How to set up Embryology laboratory-Lab space and layout, equipment, design flooring, HEPA filter etc.
- Monitoring, calibration and maintenance of equipment-measuring and adjustment of carbon dioxide (CO2) & temperature of incubators and cleaning of incubators.
- Embryology lab maintenance-daily, weekly and monthly.
- List and selection of disposables and culture media.
- Preparation of Embryology Lab for oocyte retrieval the day before-checklist.
- Preparation of lab on the day of oocyte retrieval.
- Identification of granulose cells, cumulus and corona cells and oocytes.
- Separation of oocytes and further culture.
- Preparation of semen for insemination of oocytes-processing of ejaculated semen.
- Method of oocyte insemination-short co-incubation, etc
- Dissection of oocytes and fertilization check.
- Criteria for fertilization check
Day – 1 Fertilization check, classification of zygotes and further culture
Day 2 – Assessment of embryo cleavage and identification of good embryos
Day 3 – Assessment of cleavage and selection of best quality embryos for transfer
Day 5/6- Blastocyst culture -technique
Embryo Transfer
- Preparation for embryo transfer.
- Selection of embryos for transfer- day 2, day3 or day5.
- Technique of embryo loading into the catheter (Demonstration)
Intracytoplasmic sperm injection (ICSI)
- Familiarization and operation of equipment (Training in the use of various brands of microscopes, Micromanipulators, micromanipulation tools, media etc).
- Setting up and alignment of micromanipulators and tools at different angles – Technique and tips.
- Preparation of oocytes for micro-injection-use of appropriate pipettes for dissection of oocytes in hyaluronidase and use of appropriate media.
- Preparation of sperm and egg dishes for micromanipulation.
- Selection and method of picking sperm-Technique and tips.
- Technique of microinjection-Tips.
Ultra-rapid Freezing Method-Vitrification
- Selection of Carriers for vitrification.
- Selection of vitrification solutions.
6 months Intensive Embryology training for beginners in IVF- ICSI and Andrology
Andrology Laboratory Techniques
- How to set up Andrology Laboratory and what are the equipment required?
- Handling, cleaning, maintenance and calibration of different equipment-Laminar Flow workstation, Microscopes, Centrifuges, etc.
- Comprehensive semen analysis-Macroscopic and Microscopic-density, motility, viability, round cell differentiation etc.
- Sperm morphology assessment according to WHO and Strict (Kruger) criteria.
- Sperm survival test.
- Semen cryopreservation-both neat and processed sample.
- Semen preparation for IUI-Classical method, Standard method and Density gradient method.
Embryology Laboratory Techniques
- How to set up Embryology laboratory-Lab space and layout, equipment, design flooring, HEPA filter etc.
- Monitoring, calibration and maintenance of equipment-measuring and adjustment of carbon dioxide (CO2) & temperature of incubators and cleaning of incubators.
- Embryology lab maintenance-daily, weekly and monthly.
- List and selection of disposables and culture media.
- Preparation of Embryology Lab for oocyte retrieval the day before-checklist.
- Preparation of lab on the day of oocyte retrieval.
- Identification of granulose cells, cumulus and corona cells and oocytes.
- Separation of oocytes and further culture.
- Preparation of semen for insemination of oocytes-processing of ejaculated semen.
- Method of oocyte insemination-short co-incubation, etc
- Dissection of oocytes and fertilization check.
- Criteria for fertilization check
Day – 1 Fertilization check, classification of zygotes and further culture
Day 2 – Assessment of embryo cleavage and identification of good embryos
Day 3 – Assessment of cleavage and selection of best quality embryos for transfer
Day 5/6- Blastocyst culture -technique
Embryo Transfer
- Preparation for embryo transfer.
- Selection of embryos for transfer- day 2, day3 or day5.
- Technique of embryo loading into the catheter (mock transfer)
Intracytoplasmic sperm injection (ICSI)
- Familiarization and operation of equipment (Training in the use of various brands of microscopes, Micromanipulators, micromanipulation tools, media etc).
- Setting up and alignment of micromanipulators and tools at different angles – Technique and tips.
- Preparation of oocytes for micro-injection-use of appropriate pipettes for dissection of oocytes in hyaluronidase and use of appropriate media.
- Preparation of sperm and egg dishes for micromanipulation.
- Selection and method of picking sperm-Technique and tips.
- Technique of microinjection-Tips.
Ultra-rapid Freezing Method-Vitrification
- Selection of Carriers for vitrification.
- Selection of vitrification solutions.
- Technique and precautions.
- Warming of vitrified embryos.
- Post-thaw assessment
Nitika Sharma
www.Indiaivf.in
E-mail: hr@indiaivf.in, nitika.sharma@indiaivf.in
Mobile: +91-7838277941
